Gregory J. Pazour, PhD


Professor
Program in Molecular Medicine
University of Massachusetts Medical School
Suite 213, Biotech II
373 Plantation Street
Worcester, MA 01605

(T) 508 856 8078
(F) 508 856 2950

Gregory.Pazour@umassmed.edu


Links:

Chlamydomonas Ciliary Proteome Website

Pazour Research Description (Official UMassMed Page)


Science Press Articles About Our Work:

Satir, P. 2010. Eyelashes Up Close. The Scientist 24(7):30-35

Ravven, W. 2009. Antenna on Cell Surface Is Key to Development and Disease. New York Times May 18

John Fleischman, J. 2008. Unexpected Discoveries, Unsuspected Connections: The Case of the Primary Cilium and Kidney Disease. American Society for Cell Biology Newsletter 31(12)17-19.

Kaaro, J. 2006. Karvan varassa. The Helsinki Daily Paper

Gardiner, M.B. 2005. The Importance of Being Cilia. Howard Hughes Medical Institute Bulletin Vol 18. No 2.

Vogel, G. 2005. Betting On Cilia. Science  310:216-218.

Stephan, M.M. 2004. Chasing the Cilium. The Scientist 18:14-16.

2000 Overlooked Cilia Key to Devastating Kidney Disorder. ASCB Pressbook 8.

Ferber, D. 2000 Defective Cilia Underlie Killer Kidney Disease. BioMedNet.


Movies:

(Note: If you have trouble opening these files in a browser, right click and save to file before opening.)

Phototaxis in Chlamydomonas: The movie starts with cells being unstimulated and the time is displayed at the top in hours:minutes:seconds (00:00:00). When the hours go out, the cells are being stimulated from the left, when the minutes go out, the cells are stimulated from the right. After chasing the cells back and forth, they are photoshocked a few times. Quicktime Format MPG Format


Publications:

(Note: If you have trouble opening these files in a browser, right click and save to file before opening.)

Eguether T., San Agustin J.T., Keady B.T., Jonassen J.A., Francis R., Liang Y., Tobita K., Johnson C., Hamed Z.A., Lo C.W. and Pazour G.J. 2014. IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment. Dev Cell. 2014 Nov 10.

Rao Damerla R., Gabriel G.C., Li Y., Klena N.T., Liu X., Chen Y., Cui C., Pazour G.J., Lo C.W. 2014. Role of cilia in structural birth defects: insights from ciliopathy mutant mouse models. Birth Defects Res C Embryo Today. 102(2):115-25.

Awata J., Takada S., Standley C., Lechtreck K.F., Bellvé K.D., Pazour G.J., Fogarty K.E., Witman G.B. 2014. Nephrocystin-4 controls ciliary trafficking of membrane and large soluble proteins at the transition zone. J Cell Sci. Aug 22. pii: jcs.155275. PMCID: PMC4215714.

Greer Y.E., Westlake C.J., Gao B., Bharti K., Shiba Y., Xavier C.P., Pazour G.J., Yang Y., Rubin J.S. 2014. Casein Kinase 1 Delta Functions at the Centrosome and Golgi to Promote Ciliogenesis. Mol Biol Cell. 25:1629-40. PMCID: PMC4019494.

Crouse J.A., Lopes V.S., Sanagustin J.T., Keady B.T., Williams D.S., Pazour G.J. 2014. Distinct functions for IFT140 and IFT20 in opsin transport. Cytoskeleton. 71(5):302-10. PMCID: PMC4173073.

Follit J.A., San Agustin J.T., Jonassen J.A., Huang, T., Rivera-Perez, J.A., Tremblay, K.D. and Pazour G.J. 2014. Arf4 is required for mammalian development but dispensable for ciliary assembly. PLoS Genetics 10(2):e1004170. PMCID: PMC3930517.

Finetti F., Patrussi L., Masi G., Onnis A., Galgano D., Lucherini O.M., Pazour G.J., Baldari C.T. 2014. Immune synapse targeting of specific recycling receptors by the intraflagellar transport system. J Cell Sci. 127:1924-37. PMCID: PMC4004972.

Follit J.A., and Pazour G.J. 2013. Analysis of ciliary membrane protein dynamics using SNAP technology. Methods Enzymol. 524:195-204. PMCID: PMC3844868.

Cui C., Chatterjee B., Lozito T.P., Zhang Z., Francis R.J., Yagi H., Swanhart L.M., Sanker S., Francis D., Yu Q., San Agustin J.T., Puligilla C., Chatterjee T., Tansey T., Liu X., Kelley M.W., Spiliotis E.T., Kwiatkowski A.V., Tuan R., Pazour G.J., Hukriede N.A., Lo C.W. 2013. Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton. PLoS Biol. Nov;11(11):e1001720. PMCID: PMC3841097.

Ma M., Tian X., Igarashi P., Pazour G.J., and Somlo S. 2013. Loss of cilia suppresses cyst growth in genetic models of autosomal dominant polycystic kidney disease. Nature Genetics 45(9):1004-12. PMCID: PMC3758452.

Schmidts M., Frank V., Eisenberger T., Al Turki S., Bizet A.A., Antony D., Rix S., Decker C., Bachmann N., Bald M., Vinke T., Toenshoff B., Di Donato N., Neuhann T., Hartley J.L., Maher E.R., Bogdanovic R., Peco-Antic A., Mache C., Hurles M.E., Joksic I., Guc-Scekic M., Dobricic J., Brankovic-Magic M., Bolz H.J., Pazour G.J., Beales P.L., Scambler P.J., Saunier S., Mitchison H.M., and Bergmann C. 2013. Combined NGS Approaches Identify Mutations in the Intraflagellar Transport Gene IFT140 in Skeletal Ciliopathies with Early Progressive Kidney Disease. Hum Mutat. 34(5):714-24. PMCID: PMC4226634.

Gu W., Lee H-C, Chaves D., Youngman E.M., Pazour G.J., Conte D., and Mello C.C. 2012. CapSeq and CIP-TAP map 5’ ends of Pol II transcripts and reveal capped-small RNAs as C. elegans piRNA precursors. Cell 151(7):1488-500. PMCID: PMC3581324.

Engel B.D., Ishikawa H., Wemmer K.A., Geimer S., Wakabayashi K., Hirono M., Craige B., Pazour G.J., Witman G.B., Kamiya R., and Marshall W.F. 2012. The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol. 199(1):151-67. PMCID: PMC3461521.

Keady B.T., Samtani R., Tobita K., Tsuchya M., SanAgustin J.T., Follit J.A., Jonassen J.A., Subramanian R., Lo C.W., and Pazour G.J. 2012. IFT25 Links the Signal-Dependent Movement of Hedgehog Components to Intraflagellar Transport. Dev Cell. 22(5):940-51. PMCID: PMC3366633.

Jonassen J.A., SanAgustin J., Baker S.P., and Pazour G.J. 2012. Disruption of IFT complex A causes cystic kidneys without mitotic spindle misorientation. J Am Soc Nephrol. 23(4):641-51.PMCID: PMC3312512.

Hom E.F., Witman G.B., Harris E.H., Dutcher S.K., Kamiya R., Mitchell D.R., Pazour G.J., Porter M.E., Sale W.S., Wirschell M., Yagi T., King S.M. 2011. A unified taxonomy for ciliary dyneins. Cytoskeleton (Hoboken). 68(10):555-65. PMCID: PMC3222151.

Amador-Arjonaa A., Elliota J., Miller A., Pazour G.J., Enokolopov G., Roberts AJ and Terskikha A.V. 2011. Primary Cilia Regulate Proliferation of Amplifying Progenitors in Adult Hippocampus: Implications for Learning and Memory. J Neuroscience. 31(27):9933-44. PMCID: PMC3758574.

Keady, B.K., Le, Y.Z., and Pazour G.J. 2011. Lack of IFT20 Causes Cone Cell Degeneration. Mol. Biol. Cell. Apr;22(7):921-30. PMCID: PMC3069017.

Cui, C., Chatterjee, B., Francis, D., Yu, Q., Sanagustin, J.T., Francis, R., Tansey, T., Charisse, H., Wang, B., Lemley, B., Pazour, G.J., and Lo, C.W. 2010. Disruption of Mks1 localization to the mother centriole causes cilia defects and developmental malformations in Meckel-Gruber syndrome. Disease Models & Mechanisms 4(1):43-56.

McDermott K.M., Liu B.Y., Tlsty T.D., and Pazour G.J. 2010. Primary Cilia Regulate Branching Morphogenesis During Mammary Gland Development. Current Biology 20(8):731-737.

Follit, J.A., Li, L., Vucica, Y., and Pazour, G.J. 2010. The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence. J. Cell Biol. 188:21-8.

Finetti, F., Paccani, S.R., Riparbelli, M.G., Giacomello, E., Perinetti, G., Pazour, G.J., Rosenbaum, J.L., and Baldari, C.T. 2009. Intraflagellar transport is required for polarized recycling of the TCR/CD3 complex to the immune synapse. Nat Cell Biol. 11(11):1332-9.

Follit, J.A., Xu, F., Keady, B.T., and Pazour, G.J. 2009. Characterization of mouse IFT complex B. Cell Motil Cytoskeleton 66(8):457-68.

Clement, C.A., Kristensen, S.G., Møllgård, K., Pazour, G.J., Yoder, B.K., Larsen, L.A., and Christensen, S.T. 2009. The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. J Cell Sci. 122(Pt 17):3070-82.

Follit, J.A., SanAgustin, J.T., Xu, F., Jonassen, J., and Pazour, G.J. 2008. The golgin GMAP210/TRIP11 anchors IFT20 to the Golgi complex. PLoS Genetics 4(12):e1000315.

Jonassen, J.A., SanAgustin, J., Follit, J.A., and Pazour, G.J. 2008. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J. Cell Biol. 183:377-84.

Pazour, G.J. and Bloodgood, R.A. 2008. Targeting proteins to the ciliary membrane. Current Topics in Mammalian Development 85:111-145.

Pazour, G.J. and Witman, G.B. 2008. The Chlamydomonas flagellum as a model for human ciliary disease. In: The Chlamydomonas Sourcebook, Second Edition. Vol. 3. Cell Motility and Behavior (ed. GB Witman). Elsevier, New York, NY. pp. 445-478.

Luby-Phelps, K., Fogerty, J., Baker, S.A., Pazour, G.J., and Besharse, J.C. 2008. Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors. Vision Res. 48:413-23.

Huang, K., Diener, D.R., Mitchell, A., Pazour, G.J., Witman, G.B., and Rosenbaum, J.L. 2007. Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. J Cell Biol. 179:501-14.

Tanner, C.A., Rompolas P., Patel-King, R.S., Gorbatyuk, O., Wakabayashi, K., Pazour, G.J., and King, S.M. 2008. Three members of the LC8/DYNLL family are required for outer arm dynein motor function. Mol. Biol. Cell. 19:3724-34.

Merchant et al., 2007. The Chlamydomonas genome reveals the evolution of key animal and plant functions” Science. 318:245-250.

Palenik et al. 2007 Tiny eukaryotes provide genomic insights in the paradox of the plankton” Proc. Nat. Acad. Sci. USA. 104:7705-10.

Monstant et al. 2007. Identification and comparative genomic analysis of signaling and regulatory components in the diatom Thalassiosira pseudonana. J. Phycology. 43:585-604.

Hou Y., Qin, H., Follit, J.A., Pazour, G.J., Rosenbaum, J.L., and Witman, G.B. 2007. Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. J. Cell Biol. 176:653-65.

Follit, J.A., Tuft, R.A., Fogarty, K.E., and Pazour, G.J. 2006. The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly. Mol. Biol. Cell 17:3781-3792.

Gunay-Aygun, M., Avner, E.D., Bacallao, R.L., Choyke, P., Flynn, J., Germino, G.G., Guay-Woodford, L., Harris, P., Heller, T., Ingelfinger, J., Kaskel, F., Kleta, R., LaRusso, N.F., Mohan, P., Pazour, G.J., Shneider, B., Torres, V.E., Wilson, P., Zak, C., Zhou, J., and Gahl, W.A. 2006. Autosomal recessive polycystic kidney disease and congenital hepatic fibrosis: summary statement of a first National Institutes of Health/Office of Rare Diseases conference. J. Pediatrics 149:159-164.

Fliegauf, M., Horvath, J., Schnakenburg, C.v., Muller, D., Schermer, B., Pazour G.J., Neumann, H.P.H., Benzing, T., and Omran H. 2006. Specific localization of nephrocystin at the transition zone in renal and respiratory cilia. J. Am. Soc. Nephrol. 17:2424-2433.

Yang P., Diener D.R., Yang C., Kohno T., Pazour G.J., Dienes J.M., Agrin N.S., King S.M., Sale W.S., Kamiya R., Rosenbaum J.L., and Witman G.B. 2006. Radial spoke proteins of Chlamydomonas flagella. J. Cell Sci. 119:1165-1174.

Schneider, L., Clement, C.A., Teilmann, S.C., Pazour, G.J., Hoffmann, E.K., Satir, P., and Christensen, S.T. 2005. PDGFRaa signalling is regulated through the primary cilium in fibroblasts. Curr. Biol., 15:1862-1866.

DiBella, L.M., Gorbatyuk, O., Sakato, M., Wakabayashi, K., Patel-King-R.S., Pazour, G.J., Witman, G.B., and King, S.M. 2005. Differential light chain assembly influences outer arm dynein motor function. Mol.Biol. Cell. 16:5661-5674.

Pazour, G.J., Agrin, N., Leszyk, J., and Witman, G.B. 2005. Proteomic analysis of a eukaryotic cilium. J. Cell Biol. 170:103-113.

Pazour, G.J., Agrin, N., Walker, B.L., and Witman, G.B. 2006. Identification of predicted outer dynein arm genes - candidates fro primary ciliary dyskinesia genes. J. Med. Genet. 43:62-73. Supplemental Data

Teilmann, S.C., Byskov, A.G., Pedersen, P.A., Wheatley, D.N., Pazour. G.J., and Christensen, S.T. 2005. TRP ion channels PKD1/2 and TRPV4 localize to primary and motile cilia in the female reproductive organs and the level of PKD1/2 is elevated in oviduct cilia upon ovulation. Mol. Reprod. Dev. 71:444-452.

Cano, D.A., Murcia, N.S., Pazour, G.J., and Hebrok, M. 2004. orpk mouse model of polycystic kidney disease reveals essential role of primary cilia in pancreatic tissue organization. Development 131:3457-67.

Sun, Z., Amsterdam, A., Pazour, G.J., Cole, D.G., Miller, M.S., and Hopkins, N. 2004. A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney. Development 131:4085-93.

Jurcyk, A., Gromley, A., Redick, S., San Agustin, J., Witman, G., Pazour, G.J., and Doxsey, S. 2004. Pericentrin is required for primary cilia assembly and anchoring intraflagellar transport proteins at basal bodies. J. Cell Biol. 166:637-43.

Baker, S.C., Pazour, G.J., Witman, G.B., and Besharse, J.C. 2004. Photoreceptors and intraflagellar transport. In Photoreceptors. Edited by David Williams.

Hou, Y., Pazour, G.J., and Witman, G.B. 2004. A dynein light intermediate chain, D1bLIC, is required for retrograde intraflagellar transport (IFT). Mol. Biol. Cell. 15:4382-94.

Pazour, G.J. 2004. Comparative Proteomics: Prediction of the ciliary and basal body proteome. Curr. Biol. 14:R575-7.

DiBella, L.M., Sakato, M., Patel-King, R.S., Pazour, G.J., and King, S.M. 2004. The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation. Mol. Biol. Cell. 15:4633-46.

Armbrust et. al., 2004. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306:79-86.

Pazour, G.J. 2004. Intraflagellar transport and cilia-dependent renal disease: The ciliary hypothesis of polycystic kidney disease. J. Am. Soc. Nephrol. 15:2528-36.

Wirschell M., Pazour G.J., Yoda A., Hirono M., Kamiya R., and Witman G.B. 2004. Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell. 15:2729-2741.

Baker, S.A., Freeman, K., Luby-Phelps, K., Pazour, G.J., and Besharse, J.C. 2003. IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia. J. Biol. Chem. 278:34211-34218.

Pazour, G.J., and Witman, G.B. 2003. The vertebrate primary cilium is a sensory organelle. Curr. Opin. Cell Biol. 15:105-110.

Besharse, J.C., Baker, S.A., Luby-Phelps, K., and Pazour, G.J. 2003. Phoreceptor intersegmental transport and retinal degeneration: A conserved pathway common to motile and sensory cilia. In Retinal Degeneration Advances in Experimental Biology and Medicine 533:157-164.

Casey, D.M., Inaba, K., Pazour, G.J., Takada, S., Wilkerson, C.G., Kamiya, R., and Witman, G.W. 2003. DC3, the 21-kD subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol. Biol. Cell 14:3650-3663.

Pazour, G.J., Baker, S.A., Deane, J.A., Cole, D.G., Dickert, B.L., Rosenbaum, J.L., Witman, G.B., and Besharse, J.C. 2002. The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance. J. Cell Biol. 157:103-113.

Pazour, G.J., San Agustin, J.T., Follit, J.A., Rosenbaum, J.L., and Witman, G.B. 2002. Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney disease. Current Biol. 12:R378-R380. Supplemental Data.

Pazour, G.J., and Rosenbaum, J.L. 2002. Intraflagellar transport and cilia-dependent diseases. Trends Cell Biol. 12:551-555.

Pazour, G.J., and S. Purton, S. 2002. Meeting Report: Tenth International Conference on the Cell and Molecular Biology of Chlamydomonas. Protist 153:325-336.

Pazour, G.J., Dickert, B.L. Rosenbaum, J.L., Witman, G.B., and Cole, D.G. 2000. Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene Tg737, are required for assembly of cilia and flagella. J. Cell Biol. 151:709-718.

Pazour, G.J., and Witman, G.B. 2000. Forward and reverse genetic analysis of microtubule motors in Chlamydomonas. Methods 22:285-298.

Pazour, G.J., Dickert, B.L., and Witman, G.B. 1999. The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. J. Cell Biol. 144:473-481.

Horst, C.J., Fishkind, D.J., Pazour, G.J., and Witman, G.B. 1999. An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning. Cell Motil.Cytoskeleton 44:143-154.

Pazour, G.J., Koutoulis, A., Benashski, S.E., Dickert, B.L., Sheng, H., Patel-King, R.S., King, S.M., and Witman, G.B. 1999. LC2, the Chlamydomonashomologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly. Mol. Biol. Cell. 10:3507-3520.

Pazour, G.J., Wilkerson, C.G., and Witman, G.B. 1998. A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT). J. Cell Biol. 141:979-992.

Koutoulis, A., Pazour, G.J., Wilkerson, C., Inaba, K., Sheng, H., Takada, S., and Witman, G.B. 1997. The Chlamydomonas ODA3 gene encodes a protein of the outerdynein arm docking complex. J. Cell Biol. 137:1069-1080.

Pazour, G.J., Sineshchekov, O., and Witman, G.B. 1995. Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J. Cell Biol. 131:427-440.

Moss, A., Pazour, G.J., and Witman, G.B. 1995. Assay of Chlamydomonas phototaxis. Meth. Cell Biol. 47:281-287.

Wilkerson,C., King, S., Koutoulis, A., Pazour, G.J., and Witman, G.B. 1995. The 78,000 MR intermediate chain of Chlamydomonas outer arm dynein is a WD-repeat protein required for arm assembly. J. Cell Biol. 129:169-178.

Pazour, G.J., Ta, C., and Das, A. 1992. Constitutive mutants of Agrobacterium tumefaciens transcriptional activator virG. J. Bacteriol. 174:4169-4174.

Pazour, G.J., Ta, C., and Das, A. 1991. Mutants of Agrobacterium tumefaciens with elevated vir gene expression. Proc. Nat. Acad. Sci., USA. 88:6941-6945.

Mersereau M., Pazour, G.J., and Das, A. 1990. Efficient transformation of Agrobacterium tumefaciens by electroporation. Gene. 90:149-151.

Pazour, G.J., and Das, A. 1990. VirG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence specific DNA binding protein. J. Bacteriol. 172:1241-1249.

Pazour, G.J., and Das, A. 1990. Characterization of the VirG binding site of Agrobacterium tumefaciens. Nucl. Acids. Res. 18:6909-6913.

Sen, P., Pazour, G.J., Anderson, D., and Das, A. 1989. Cooperative binding of Agrobacterium tumefaciens VirE2 protein to single-stranded DNA. J. Bacteriol. 171:2573-2580.

Das, A., and Pazour, G.J. 1989. Delineation of the regulatory sequences of Agrobacterium tumefaciens virB operon. Nucl. Acids Res. 17:4541-4550.

Disclaimer: This is an unofficial UMMS page.