Oligonucleotide Microarrays

Oshima T, Wada C, Kawagoe Y, Ara T, Maeda M, Masuda Y, Hiraga S, Mori H. (2002. Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli. Molecular Microbiology 45, 673-695) have measured transcriptional activity in dam mutants grown under a variety of conditions using high density oligonucleotide arrays.


Løbner-Olesen, A., Marinus, M.G. and Hansen, F.G. 2003. Role of SeqA and Dam in Escherichia coli gene expression- a global/microarray analysis. Proc.Natl.Acad.Sci. USA, 100, 4672-4677) have measured global transcription in seqA, dam, aroK and Dam-overproducing strains using Affymetrix gene chips. The results are contained in the files below and can be accessed as either plain text (txt) or Excel (xls) files.

MG1655 [txt] [xls]
KS9921 (MG1655 seqA) [txt] [xls]
ALO1738 (MG1655 dam) [txt] [xls]
ALO1739 (MG1655 aroK) [txt] [xls]
ALO1740 (MG1655/pTP166) [txt] [xls]

Robbins-Manke JL, Zdraveski ZZ, Marinus M, Essigmann JM. 2005. Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli. J Bacteriol. 187, 7027-37.) measured global transcription in dam, dam mutS and mutS strains. (Files are in PDF format)

AB1157
GM3819
GM5555
GM5556

Campellone KG et al 2007. Increased adherence and actin pedestal formation by dam-deficient enterohemorrhagic Escherichia coli O157:H7. Molec. Microbiol. 63, 1468-1481.

EHEC dam


Løbner-Olesen, A., Slominska-Wojewodzka, M., Hansen, F.G., and Marinus, M.G. 2008 DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes. Submitted for publication.

DnaC


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