pALO160 AmpR; A very low
copy plasmid with a 7.8 kb BglII insert of chromosomal DNA
including the
aroK, aroB, urf74, and dam genes. (GM5488)
Lobner-Olesen et al 1992 Mol Microbiol 6, 1841-1851
pALO163 AmpR; As pALO160
but containing
a lacZ transcriptional fusion in the unique BamHI site
in the
dam gene
Lobner-Olesen et al 1992 Mol Microbiol 6, 1841-1851
pALO218 AmpR; As pALO163
but containing
the CamR gene from pACYC184 (on a 1.4 kb BsaAI
fragment)
cloned into the NruI site of aroK (GM5627)
Lobner-Olesen A. and Marinus MG 1992 J Bacteriol.,
174 ,525-529.
pBA40 AmpR; BglII
fragment of chromosome with the mutS
gene
cloned into the BamHI site of pBR322 (GM5612).
Obtained from Dr. Ken Stacey, University of Canterbury, UK
Wu TH and Marinus MG 1994 J. Bacteriol.
176,
5393-5400
pJEL109 AmpR; A very low copy plasmid with an
R1
origin of replication, the bla gene of Tn3 and cloning
sites
for BamH1, ClaI and HindIII.
Parent plasmid of pALO160. Constructed by JEL Larson and cited in
Lobner-Olesen et al 1992 Mol Microbiol 6, 1841-1851. (GM5490)
pMQ3 AmpR; 12.1 kb plasmid containing a 6.1 kb EcoRI
dam fragment cloned into pBR325.
(GM1235)
Arraj JA Marinus
MG 1983 J.
Bacteriol. 153, 562-565
pMQ133 CamR; 6.8 kb plasmid containing a 1.9 kb PstI
CamR fragment from Tn9; a 1.8 kb PstI-SalI
dam fragment and the SalI-PstI
backbone of pBR322. (GM2284)
Arraj JA Wu TH
Marinus MG 1990
Current Microbiol. 20, 133-136
pMQ148
AmpR; Identical to
pdam118 (Brooks et al 1983 Nucleic
Acids Res 11,
837-851). Contains a pBR322 duplication. Unlike other dam
plasmids
produces only a two-fold increase in cellular Dam activity.
(GM2374)
Arraj JA Wu TH
Marinus MG 1990
Current Microbiol. 20, 133-136
pMQ191 TetR; EcoRI - EcoRI (destroyed PvuII plus EcoRI linker) dam fragment from pTP166 into pACYC184 EcoRI site. (GM3166)
pMQ269 AmpR,
TetR ; pPY97 derivative with T instead of C at nt 40
altering the
His6 codon of mnt.
For preparation of G-T mismatch at nt 40
pMQ270 AmpR,
TetR ; pPY97 derivative with G instead of A at nt 41
altering the
His6 codon of mnt.
For preparation of T-G mismatch at nt 41
pMQ271 AmpR, TetR ; pPY97 derivative with 1 bp deletion (-A41) altering the His6 codon of mnt. (GM5421)
pMQ272 AmpR, TetR ; pPY97 derivative with 2 bp deletion (-GC, 39-40) altering the His6 codon of mnt. (GM5422)
pMQ273 AmpR, TetR ; pPY97 derivative with 3 bp deletion (-GCA, 39-41) altering the His6 codon of mnt. (GM5423)
pMQ274 AmpR, TetR ; pPY97 derivative with 4 bp deletion (-CGCA, 38-41) altering the His6 codon of mnt. (GM5424)
pMQ275 AmpR, TetR ; pPY97 derivative with 5 bp deletion (-GCACT, 39-42) altering the His6 codon of mnt. (GM5425)
(Plasmids pMQ269-pMQ275) Parker BO and Marinus MG
1992 Proc Natl
Acad Sci
USA. 89, 1730-1734
Carraway M and marinus MG 1993 J. Bacteriol. 175,
3972-3980.
pMQ315
AmpR, mutS+;
derived
from pBA40 by MNNG mutagenesis of region upstream of mutS.
(GM5782)
Wu TH and Marinus MG 1994 J.
Bacteriol.
176, 5393-5400
pMQ339 CamR; pACYC184 with a BamHI
chromosomal
DNA fragment containing the mutL
gene
cloned into the BamHI site (GM6494)
Wu TH and Marinus MG 1994 J.
Bacteriol.
176, 5393-5400
pMQ341
CamR; pACYC184 with a
replacement ClaI-HindIII fragment from pBA40 containing
the mutS gene (GM6497)
Wu TH and Marinus MG 1994 J.
Bacteriol.
176, 5393-5400
pMQ348
CamR; pACYC184
with a
XhoI-HindIII fragment from pRH71-17 containing the mutH gene replacing the SalI-HindIII
sequence (GM6470)
Wu, T.-H. and Marinus, M.G. (1994) J.
Bacteriol.
176, 5393-5400
pMQ350 AmpR; pZ150 with BamHI
chromosomal mutL fragment (GM6509)
Aronshtam, A. and Marinus, M.G. 1996. Nucleic Acids
Research 24,
2498-2504
-this plasmid may be unstable generating deletions
in
the mutL gene
pMQ372 AmpR; pET3d vector
(plus destroyed AvaI site) with mutS
gene (GM6593) [Map]
Deletion derivatives
pΔ1-260 (GM6928)
pΔ26-260 (GM6827)
pΔ261-556 (GM6833)
pΔ680-853
(GM6825)
pMQ382 CamR; pACYC184 expressing His-Tagged MutS from a T7 promoter. (GM6627) [Map]
pMQ393 CamR; pACYC184
expressing His-Tagged MutL from a T7
promoter. (GM6647)
Aronshtam A. and Marinus MG 1996. Nucleic Acids
Research 24,
2498-2504
pMQ395 AmpR; pMQ372
derivative with N-terminal His Tag of pET15b fused to produce
His-Tagged MutS (GM6651)
[Map]
Deletion derivatives
pΔ1-311H
(GM6922)
pΔ26-260H
(GM6425)
pΔ261-556H (GM6927)
pΔ680-853H (GM6924)
Wu TH and Marinus MG 1999 JBC 274, 5948-5952
pMQ402 AmpR; pBAD18 expressing His-Tagged MutH from the araBAD promoter. XbaI-HindIII mutH fragment inserted into pBAD18. (GM6851)
pMQ417 AmpR; pMQ372
derivative with C-terminal His Tag fused to produce His-Tagged MutS (GM6929)
The MutS protein encoded by this plasmid is fully functional and is
more
stable in vitro than that produced by pMQ395.
Wu, T.H. and Marinus, M.G., unpublished data.
pMQ430 AmpR; pBAD18
derivative with the cloned dam
gene, without its ribosome binding site, under control of the pBAD
promoter. Expression of dam
occurs in the presence of arabinose but not
in its absence.(GM7470)
Calmann MA and Marinus MG 2003 J. Bacteriol. 185,
5012-5014.
pMS2 AmpR; carries
the wildtype
aroK and aroB
genes. A pBR322 derivative in which the AseI-EcoRI
fragment has been replaced with the corresponding fragment from
pALO160. (GM5770)
Lobner-Olesen A. and Marinus, MG 1992 J Bacteriol.,
174 ,525-529.
pN3 TetR StrR SulR (use 30, 50,
100 ug/ml). This plasmid bears the EcoRII
restriction/modification system.
See Watanabe
et al 1964 and Hattman et al 1972.
pPY97 AmpR; mnt-Pant::tet,in a pBR322 (M13ori, +) backbone.
pPY98 AmpR; mnt-Pant::tet,in a pBR322 (M13ori, -) backbone.
pRH71-17 (mutH+
in a pBR322 backbone, AmpR). Plasmid obtained from Dr R.
Grafstrom. (GM4151)
Grafstrom RH Hoess RH Gene 1983 22:245-53
Grafstrom RH Hoess RH Nucleic Acids Res 1987
15:3073-84
pTP166 AmpR; dam gene under tac promoter control.
(GM2290)
Marinus MG Poteete A Arraj JA l984 Gene
28:123-l25
