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The propagation of
an intercellular Ca2+ wave from astrocytes to
endothelial cells Initiation of an intercellular Ca2+ wave by mechanical stimulation (arrow) in a glial cell, that propagates through glial cells and into adjacent endothelial cells in co-culture. White line indicates endothelial-glia interface. Figure 1; Leybaert et al., (1998), Glia, 24, 398 - 407. Time between images = 0.3 seconds. File Size: 553 Kb |
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Intercellular
Ca2+
waves initiated in astrocytes by flash photolysis of
caged IP3 Sequence 1, First Arrow: Photolysis of the cell initially injected with caged IP3. Sequence 2, Second Arrow: Flash photolysis of a distal cell that received caged IP3 by intercellular diffusion via gap junctions. Figure 2; Leybaert et al., Glia, 24, 398 - 407. Time between images = 0.3 seconds. File Size: 626Kb |
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Changes in
[Ca2+]i induced by photolysis
of caged calcium (NP_EGTA) |
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An intercellular
calcium wave generated by mechanical stimulation of the
same cell shown in Figure 4a. The presence of NP-EGTA does not effect wave propagation. Figure 4b; Leybaert et al., (1998), Glia, 24, 398 - 407. File Size: 210K |
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An intercellular
Ca2+ wave initiated by flash photolysis of caged IP3 that
propagates from endothelial cells to astrocytes Black Arrow: Glial cell injected with caged IP3; White Arrow: Endothelial cell flashed with UV light. Figure 5: Leybaert et al., (1998), Glia, 24, 398-407. Time between images = 0.3seconds. File Size: 508 Kb |
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A 3-D reconstruction of gap junctions and
endoplasmic reticulum in HeLa cells. The sequence shows a rotating 3D image of two adjacent cells. The gap junctions are shown in red and were visualized by transfecting HeLa cells with constructs for Cx43 labeled with GFP. The ER is shown in green and was identified with a live-cell stain ER-tracker (Molecular Probes). Magnification Image width and height = 43 um. File Size: 2.8 Mb. Paemeleire et al., Mol. Biol. Cell. (2000) In Press. |
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An intercellular calcium wave propagating via
a gap junction between two HeLa cells (seen in 3D). A Ca2+ wave moving through two adjacent cells. Increasing brightness represents an increase in [Ca2+]i. The fluorescence at the site of the gap junctions does not change. The Ca2+ wave was initiated by mechanical stimulation in the cell in the upper left and appears to be initiated from the the site of the gap junction. The role of the extracellular messenger ATP, was ruled out by the presence of apyrase. Time between images = 33 ms. Magnification Image width and height = 43 um. File Size: 1.8 Mb. Paemeleire et al., Mol. Biol. Cell. (2000) In Press. |