| High-speed Digital images and analysis | ||
| The intensity of the microscope image varies as cilia move and this effect can be used to measure CBF. A Region of Interest (ROI, arrow) is selected over the cilia C, and the intensity changes recorded. |
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| A gray-scale waveform is obtained and each cycle represents one beat cycle. To measure CBF, a cross Line (arrow) is calculated each second that is at the mid point of the waveform. |
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The beginning of each ciliary beat cycle is determined by the point at which the positive going gray scale waveform intersects the cross line (arrows). The time between these two points is equal to the beat cycle period which is the inverse of the CBF. |
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The temporal resolution of the CBF measurement is increased by interpolating the gray scale waveform between two adjacent data points and using simple trigonometry to estimate the exact time of the cross point. |
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