| Methods
for High-Speed Digital Microscopy: For the quantification of ciliary beat frequency (J Physiology, In Press) |
|||||
| Normal video images are
formed by a series of interlaced horizontal (H) lines that scan
the scene. Each image is recorded in 1/30 second.
However, cilia often beat at frequencies approaching 30 Hz and the quantification of ciliary activity requires a faster image capture rate. We recommend a rate of about 240 fps. We have achieved this with a digital imaging system.
|
OLD System (1999): Based on conventional video and used customized equipment that was not available to everyone. NEW System (2002): Based on digital images and uses only commercially available equipment and software. |
||||
| Basic System Components | |||||
|
|
|
|
|
|
|
| A high-speed camera provides images to a frame grabber and under the control of the software Video Savant, these images are written to hard drive in real-time. |
|
||||
|
The computer used was assembled from components as this was the
easiest way to get the required configuration.
The minor components of the system are not critical. |
A
Super Micro Xeon mother board: P4DC6 with a 64 bit PCI bus and Ultra
160 SCSI ports on board.
The SCSI array was made of |
||||
