PROFESSIONAL EXPERIENCE

1991:               Teaching Assistant of Introductory course to Science Faculty. Facultad de Ciencias, UCV.

1991:               Teaching Assistant in Biostatistics. Facultad de Ciencias, UCV.

1992:               Teaching Assistant Introductory course, to Science Faculty. Facultad de Ciencias, UCV.

1992-1993:      Research assistant. Biotechnology Laboratory. Facultad de Ciencias, UCV. Proyect: “Genetic improvement of Cereals and Legumes”

1993:               Teaching Assistant of Biochemistry Laboratory. Facultad de Ciencias, UCV

1994-1995:      Research assistant. Biotechnology Laboratory. Facultad de Ciencias, UCV. Proyect: “Genetic improvement of Cereals and Legumes”

1995-1996:      Student Assistant. Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC)

1996-2001:      Graduated Student. Centro de Estudios Avanzados (CEA), IVIC. PhSc in Biochemistry.

2002-Present:  Post-doctoral Associate, University of Massachusetts, Medical School, MA. 

SELECTED PUBLICATIONS

Perez, J.F.; Ruiz, M-C and Michelangeli, F. (2001): Simultaneous measurement and imaging of intracellular Ca²⁺ and H⁺ transport in isolated rabbit gastric glands. J. Physiol. 537: 735-45.

Perez, J.F.; Ruiz, M-C; Chemello M-E; Michelangeli, F. (1999): Characterization of a membrane calcium pathway induced by rotavirus infection in culture cells. Journal of Virology 73: 2481-2490.

Perez, J. F; Chemello, M.E.; Liprandi F; Ruiz, M-C.; Michelangeli, F. (1998): Oncosis in MA104 cells is induced by rotavirus infection through an increase in intracellular Ca²⁺ concentration. Virology 252: 17-27.  

D’Suze, G.; Sevcik, C.; Perez, J. F. and Fox, J.W. (1997): Isolation and Characterization of a potent curarizing polypeptide from Tiyus discrepans scorpion venom.  Toxicon  36: 1683-1689.

Book Chapters

Perez, J.F.; M.C. Ruiz, M.C. and Michelangeli, F. (2002): Measurement of Intracellular Calcium and Gastric Acid Secretion in Real Time Using Fluorescent Probes in Molecular and Celular Mechanisms of H+ Transport (Forte, J. & Urushidani, T., ed.). Springer-Verlag Berlin, Heidelberg. In press.

 Michelangeli, F.; Perez, J.F. and Ruiz, M.C. (2002): Kinetics of Activation of H+ Secretion by Secretagogues in Relation to Changes in [Ca2+]i in Isolated Gastric Glands in Molecular and Celular Mechanisms of H+ Transport (Forte, J. & Urushidani, T., ed.). Springer-Verlag Berlin, Heidelberg. In press.

 Recent Abstracts

 Perez, J.F.; Ruiz, M-C and Michelangeli, F. (2001):  A new method to determine gastric acid secretion in isolated gastric glands with spatio-temporal resolution using (the fluorescent probe) lysosensor yellow-blue. IUPS Congress 2001, Christchurch, New Zealand.

Michelangeli, F. Perez, J.F. and Ruiz, M.C. (2001): Simultaneous measurement and imaging of intracellular Ca2+ and H+ transport in isolated gastric glands following stimulation with secretagoges. IUPS Congress 2001, Christchurch, New Zealand.